- Argall’s Gift: Thomas Pawlett, Edward Gourgaing
- Charles City: Samuel Sharpe, Samuel Jordan
- Flowerdieu Hundred: Edmund Roffingham, John Jefferson
- Henricus: Thomas Dowse, John Polentine [probably Pollington]
- James City: William Powell, William Spense (Spence)
- Kiccowtan: William Tucker, William Capp
- Lawne’s Plantation: Christopher Lawne, Ensign Washer
- Martin’s Brandon: * Thomas Davis, Robert Stacy
- Martin’s Hundred: John Boys, John Jackson
- Captain Warde’s Plantation: John Warde, John Gibbes
- Smythes Hundred: Thomas Graves, Walter Shelley
* The representatives form this plantation were not allowed to serve.